Ethan Strattan

4 Questions 2 Answers 0 Followers

Questions related from Ethan Strattan

Golgistop (monensin) before LPS stimulation in mast cells?

I'm currently trying to stain for intracellular cytokines in mast cells, and have been having trouble getting my stains to show up by flow. I know that these cytokines are being produced, as...

05 July 2017 6,281 2 View

Why am I not getting mast cells from murine BMMC culture?

http://gallilab.stanford.edu/protocols/documents/1BMCMCculture.pdf I've been following the protocol above, but in short: I extracted bone marrow from mice, washed the cells, and resuspended in...

17 April 2017 1,524 3 View

What is the best way to get binding site multimers for a reporter assay?

I'm looking to do a luciferase reporter assay with a mutated and unmutated version of the binding sites for my GOI. I want to get a multimeric binding site cloned in, preferably 4-5 repeats of an...

02 July 2015 7,522 4 View

Do I need to keep cell lines under selection after they have grown out post-transduction?

After selection, when cells have grown out in the presence of my antibiotic, do I keep them under selection with antibiotic indefinitely, or take them off?

30 March 2015 7,839 4 View