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Questions related from Ethan McKee
I designed primer and probe sets for two separate regions. The regular PCR was successful but my qpcr resulted in these abnormal curves. Does anyone know what could be the issue?
02 July 2025 2,841 3 View
I've started doing sanger sequencing recently and only rarely get good results. Every run I do barely has >100 Q20 and always has weak signal and excessive background noise. I've attached an...
11 November 2022 2,399 2 View
Hello, I've been tasked with validating a 2kb region of DNA. A colleague designed primers with the F melting at 53.8 and the reverse at 59.7. I believe they used 54C which had multiple bands show...
17 October 2022 5,448 6 View
