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I've started doing sanger sequencing recently and only rarely get good results. Every run I do barely has >100 Q20 and always has weak signal and excessive background noise. I've attached an...
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Hello, I've been tasked with validating a 2kb region of DNA. A colleague designed primers with the F melting at 53.8 and the reverse at 59.7. I believe they used 54C which had multiple bands show...
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