Esther Senden

4 Questions 1 Answers 0 Followers

Questions related from Esther Senden

Multiple comparison with two or more factors after perMANOVA?

Hi, Does anybody have experience with post-hoc test comparing two factors after perMANOVA? I have Plant_ID : 3 levels and within each Plant_ID there is Petal_Type with 2 levels. I want to know...

01 October 2020 2,176 3 View

How to do post-hoc and separate between main effects on multiple dependent variable using PerMANOVA test?

Hi, I am trying to test the effects of Plant ID (Different flower colours) and Petal Type (each flower was divided into two different petal types) on 5 different pigment concentration...

01 October 2020 1,110 2 View

How do I get rid of DNA contamination when DNase and exon-spanning primers don't work?

I'm working on a plant species without a reference genome, I do have transcriptome data available (de-novo assembly). Now I want to do qPCR on a group of genes that were differentially expressed...

12 July 2020 4,457 0 View

How to design primers for unique region in a multiple sequence alignment?

I am looking for a specific gene in my transcriptome data of a non-model species. I aligned all transcripts that were annotated to this particular gene. Now I want to design primers for one...

15 July 2019 4,591 3 View