Estefania Elorriaga

2 Questions 6 Answers 0 Followers

Questions related from Estefania Elorriaga

Should I rehydrate frozen pollen before extracting RNA? or before germinating it in-vitro?

Hello. I will be extracting RNA from frozen eucalypt pollen soon. It has been in the -80 freezer for over a year. Should I rehydrate it before I extract RNA? I will also be germinating some...

05 May 2017 3,694 4 View

How can I get rid of smear around PCR band?

Hello, I amplified a gene that I plan of sequencing using TOPOcloning TA. I was wondering what changes could I make to reduce the amount of smearing (I attached the gel photo). I do have a long...

10 October 2016 5,531 11 View