Esraa Mohsen

4 Questions 3 Answers 0 Followers

Questions related from Esraa Mohsen

Flowcytometry techniques for detection of cell surface and intracellular proteins?

Please, I need a detailed flowcytometry monoclonal antibodies protocol for detection of TLR4(cell surface) and TLR3, TLR7 (intracellular) using commonly used buffers as staining, permeabilization,...

26 November 2024 6,288 1 View

Re perform PCR reaction on the same first one for genotyping?

I performed genotyping analysis. Some samples give a result of one SNP but didnt give a result for another SNP. Note, these SNPs are performed at the same plate (same condition). Depending on the...

27 March 2024 7,079 0 View

Can I store the phenol layer containing protein after rna extraction with trizole?

I want to extract RNA from blood sample and storing the phenol layer that contain proteins without processing, is this will affect the protein?

21 December 2023 6,009 3 View

Lymphocyte separation and culturing technique?

Good evening, I want to separate lymphocytes from human blood then culture these cells for stimulation and determine the fractions of T, B and NK cells in samples. Is the attached protocol is...

09 July 2023 2,707 3 View