Esra Bedir

4 Questions 11 Answers 0 Followers

Questions related from Esra Bedir

If a gene from one bacterium is to be heterologously expressed in another, is codon optimization necessary?

Hello everyone, In a study I'll be conducting, I aim to heterologously express a gene derived from Streptomyces in E. coli. I'm wondering whether codon optimization is necessary in this case....

last month 5,702 2 View

How to subculture Expi293F cells?

Hello everyone; I am trying to develop HEK293F suspension cells for recombinant protein production. I use the Expi293 Expression System kit for this. HEK293F cells were cultured in Expifectamine...

28 November 2023 9,047 6 View

What do you think is the best method for bacteriocin isolation from lactic acid bacteria?

Hi everyone, I'm working on the isolation of bacteriocin from lactic acid bacteria. I've a bacteria that I isolated and know to be lactobacillus. When I cultivated this bacterium in liquid culture...

05 March 2023 879 18 View

How can I solve my SDS-PAGE problem after ammonium sulphate precipitation?

Hello everyone; After precipitating the proteins with ammonium sulphate, we performed SDS-PAGE. However, there was a problem with the gel running, we couldn't see any tape. We did dialysis to...

14 February 2023 3,911 5 View