Eslam Katab

4 Questions 9 Answers 0 Followers

Questions related from Eslam Katab

What's a good cytosolic marker and a loading control other than Tubulin?

I am running a simple western experiment (using WES machine) where I test cytosolic versus nuclear fractions of mouse B cells (Using only 1million cells) and unfortunately Tubulin signal is not...

12 February 2019 1,172 3 View

What is the best applicable inhibitor of autophagy?

I use 3-methyl adenine at a final concentration of 7 mMol, but I am not convinced about using it because it solidifies and forms aggregates in the media.

15 February 2017 6,948 15 View

Low Conc. of maxi-prep DNA?

I am cloning different plasmids using normal cloning protocol. Then I pick colonies of this cloning and run a mini-prep. DNA of the mini is always subjected to double digest in order to check for...

24 January 2017 2,602 5 View

I did cloning using TOPO® TA Cloning® Kit with PCR®2.1 TOPO®, I inoculated 100 ul on Ampicilin plates, but nothing came.. so what could be happened?

Plates were incubated for 12 hours. Kit info: https://tools.thermofisher.com/content/sfs/manuals/topota_man.pdf

08 June 2016 1,316 6 View