Erin Analiese Hancock

2 Questions 1 Answers 0 Followers

Questions related from Erin Analiese Hancock

Do DH5a and MC4100 competent cells Fluoresce?

Hello, I have currently been tasked with performing a split GFP analysis. I have been using DH5a cells for plasmid transformation. The results were turning out to be what we expected. The...

30 May 2024 5,664 0 View

How do I prevent this deletion during ligation?

Hello, I am making some plasmid constructs via Inverse PCR and T4 enzyme ligation. I started with a plasmid that housed nucleotide sequences for two fused proteins. My goal was to cut out the...

15 May 2024 3,186 3 View