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Hello everyone! I am using the Ambion Firstchoice RLM-RACE kit to get both extremes of two mRNA from a Diatom. For the 3'-RACE i did't experience any complication, but so far I am still not able...
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I've tried many different methods, but I have no signal. My current protocol (for any other protein it works very well, but not now) is a Bis-tris gel (8%), MOPS running buffer (running at 100v),...
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