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I am following a protocol described in this paper by Ma et al: Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction. They state: Triton X-114 isothermal extraction was...
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I currently have a protein expression construct (for e coli expression) that is MBP tagged. However, the MBP tag sticks to my protein after cleavage. Therefore, I would like to express it as...
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I am cloning a fragment into my expression vector using XbaI and HindIII digestion. I linearized my vector and did gel extraction to remove the excised fragment from the vector. (The vector was...
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