Erel Hatan

7 Questions 7 Answers 0 Followers

Questions related from Erel Hatan

How to convert herbicide concentration in soil to PPM?

Hi all, I want to conduct an experiment of stability of different herbicides in soil as dependent on time. Now, I know that 1mg/ul (a.i) of herbicide in 1 kg of soil is equal to 1 PPM. my question...

12 August 2021 5,440 5 View

Recommendations for total protein content kit

Hi all. I need to perform total protein content assay on plant tissue (leaves) and I want to know if someone can point me to standard protocol and good kit for that matter. Thanks a lot Erel

01 January 1970 506 3 View

Low BSA standard curve values

Hi all. when performing Bradford assay on plant tissue, I chose BSA protein as my standard protein for calibration curve. the concentrations needed for the curve are: 2mg/ml, 1.5, 1, 0.75, 0.5,...

01 January 1970 4,303 1 View

Protein dilution for BCA assay?

Hi all. I recently changed my protein assay from Bradford to BCA because of the sensitivity of the Bradford to the detergent in my lysis buffer. The technical support of Thermo told me that no...

01 January 1970 6,430 7 View

Protein content with Bradford assay

hi all. I'm currently performing Bradford assay in 96-well plate. can I re-use the plates after each test? I'm washing the plates Thoroughly with water + Sodium hypochlorite and leave it for...

01 January 1970 4,924 5 View

IAA levels inside plants treated with 2,4-D

Hi all. I wish to know what are the effects of 2,4-D treated plants in aspect of its endogenous auxin biosynthesis (IAA- Indole-3-acetic acid)? does the exogenous artificial auxin mimic in form of...

01 January 1970 3,975 1 View

Total protein content assay

Hi all, after extracting my protein from lettuce leaves using Pierce total protein extraction kit I want to check my protein using Bradford assay, can someone please point me to the procedure I...

01 January 1970 7,820 3 View