Eray Sahin

2 Questions 1 Answers 0 Followers

Questions related from Eray Sahin

What is the reason for different fold changes in gene expression from independent experimental repeats in Real-time PCR experiments ?

Hi, I'm trying to analyze the expression changes of some genes following under stress conditions or plasmid transfection, using real-time PCR. After finding the best housekeeping candidate using...

26 October 2014 7,286 3 View

Total Protein Isolation Problem with RIPA Buffer?

I was trying to make a RIPA buffer to isolate total protein from about 10 M SH-SY5Y cells seeded onto a 150 mm-dish. For that pırpose, I mixed 50 mM Trsi-HCl (pH:7.4), 1% Triton X-100, 0.5%...

30 November 2013 517 17 View