Engi Hassaan

6 Questions 7 Answers 0 Followers

Questions related from Engi Hassaan

2 molecules in the asymmetric unit; one ordered and the other disordered?

I have a protein crystal structure at 1.83 angstroms with 2 molecules in the asymmetric unit. But the B factor of the second molecule is almost twice as large as the first. After running ARP/wARP...

30 August 2019 8,259 5 View

What is a self intensity test in STD NMR?

Some papers report performing a "STD self intensity" test to check the aggregation of compounds in STD NMR. Can anyone please elaborate how such a test is performed?

28 August 2019 3,289 2 View

How can I open a biacore BLR file without having a biacore software!?

I did some SPR experiments in another lab, but now that I am back in my own lab we do not have a biacore instrument and thus neither the software. Can anyone please recommend a software to just...

14 May 2019 1,853 6 View

What are the consequences of using very high ligand concentrations in ligand-observed NMR screens?

In our CPMG experiments, we use ligand concentrations of around 300uM. What are the possible drawbacks (besides aggregation) that could arise from using say up to 1mM of fragment?

23 January 2019 8,696 1 View

What is the maximum sample capacity for HiLoad 26/600 Superdex 75 SEC?

I read in the manual that the maximum volume is 13ml, but it does not state the maximum protein concentration. Does anyone know it? Thanks.

29 September 2017 9,462 1 View

Has anyone tried to add IPTG to auto inducing media like ZYM? Did you notice an increase in protien expression or is it pointless?

I express using BL21 DE3 e-coli cells and use the ZYM 5052 media. Some colleagues who also use this media have suggested adding IPTG to increase the protein expression. Would that make any sense...

01 July 2017 3,659 4 View