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Hi! I'm wondering if anyone else has had a situation where they are purifying an enzyme, and only one clone is active? The cloned gene is genetically identical to other clones, but only this one...
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Hi! I've been studying a particular enzyme that in in vitro experiments exhibits two optimal pH's. All other parameters are the same (incubation time, substrate, temperature, salt concentration,...
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I'm using an accumet basic AB15 pH meter to measure citrate-phosphate buffers. I accidentally re-standardized the probe using the provided pH 4.00 buffer without deleting my previous calibration....
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