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Questions related from Emma Monaghan
I'm a bit confused by my cloning PCR results. I have used the TOPO TA Cloning kit and everything seemed to go to plan - I got colonies, I grew them in the indicated liquid culture and then...
10 April 2014 4,304 6 View
I was just wondering what your thoughts were on storage of purified PCR products - fridge or freezer temperature? I always elute purified products using the buffer provided with the purification...
01 April 2014 3,117 4 View
I have read that repeated freeze-thawing can damage DNA but then a lot of kit guides recommend freezing for storage. I have stored PCR products and purified PCR products in the fridge and found...
20 November 2013 9,121 12 View
I am determining my protocol to run a denaturing DNA PAGE gel to look at DNA fragments just under 100bp. I was wondering whether people commonly used a stacking gel to enable a good transition of...
08 April 2013 8,473 3 View