Emma Monaghan

4 Questions 9 Answers 0 Followers

Questions related from Emma Monaghan

What is going on with my M13 primer PCR using TOPO TA Cloning kit produced DNA?

I'm a bit confused by my cloning PCR results. I have used the TOPO TA Cloning kit and everything seemed to go to plan - I got colonies, I grew them in the indicated liquid culture and then...

10 April 2014 4,368 6 View

What is the best storage method for purified PCR products?

I was just wondering what your thoughts were on storage of purified PCR products - fridge or freezer temperature? I always elute purified products using the buffer provided with the purification...

01 April 2014 3,162 4 View

What are your opinions on storage of PCR products and purified PCR products in terms of refrigeration vs. freezing?

I have read that repeated freeze-thawing can damage DNA but then a lot of kit guides recommend freezing for storage. I have stored PCR products and purified PCR products in the fridge and found...

20 November 2013 9,181 12 View

Denaturing DNA PAGE.

I am determining my protocol to run a denaturing DNA PAGE gel to look at DNA fragments just under 100bp. I was wondering whether people commonly used a stacking gel to enable a good transition of...

08 April 2013 8,529 3 View