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Questions related from Emma Monaghan
I'm a bit confused by my cloning PCR results. I have used the TOPO TA Cloning kit and everything seemed to go to plan - I got colonies, I grew them in the indicated liquid culture and then...
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I was just wondering what your thoughts were on storage of purified PCR products - fridge or freezer temperature? I always elute purified products using the buffer provided with the purification...
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I am determining my protocol to run a denaturing DNA PAGE gel to look at DNA fragments just under 100bp. I was wondering whether people commonly used a stacking gel to enable a good transition of...
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