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I have purchased this plasmid from addgene: https://www.addgene.org/70719/ From the agar stab, I have been able to grow the plasmid on LB-ampicillin plates (100mg/L) and it grows great. I...
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I was starting a PCR cleanup with QIAquick kit from QIAGEN, when I realized I was out of spin columns. I already added buffer PB (guanidine hydrochloride and isopropanol) and a little sodium...
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