Emily J Shultz

5 Questions 5 Answers 0 Followers

Questions related from Emily J Shultz

Can antibiotics produced in bacterial culture be visualized by native PAGE?

I would appreciate suggestions for other modes of antibiotic visualization, preferably electrophoresis or gel based.

27 September 2016 7,131 4 View

Can a Brucella abortus pathogenesis expert advise why Brucella T4SS effectors localize to Golgi if BCV associates exclusively with the ER?

I am attempting to write a grant proposal for a class that aims to look at the protein-proteins interactions of the T4SS effector BspC and Golgi proteins such as GM130. It has been shown that out...

22 November 2015 513 0 View

Can anyone with restriction enzyme choice for cloning and the effect of CpG methylation sensitivity on the enzyme NheI in my gene of interest?

I know this may seem a little silly to ask, I bet there is a straight forward answer, but I have no clue. I want to use the restriction enzyme NheI to cut my gene of interest, but it has CPG...

12 November 2015 3,534 5 View

What are the very small blurred bands at the bottom of my agarose gel?

In three of the agarose gels that I have looked at by UV, I have seen these very small blurred bands at about 100 bp, despite sample type (RNA, PCR cDNA, or cleaned up PCR cDNA), and as mentioned...

06 November 2015 9,834 3 View

How do I optimize cloning a large insert (5800 bp) into 5800 bp pET100/D-Topo vector?

So I have a MyH2 gene insert that is 5800 bp with a concentration of 40 ng/ul and a pET100/D-Topo vector with a concentration of 15-20 ng/ul. The insert was PCR amplified, cleaned using column...

03 November 2015 2,920 9 View