8 Questions 22 Answers 0 Followers
Questions related from Eljelani SALIM Salim
the attached qPCR picture shows the melting curve of my samples. the efficiency is 43.5%. I am actually targeting genomic DNA of Cryptosporidium actin gene from direct faecal extraction.
07 October 2016 8,031 5 View
can anybody suggest why qPCR reaction dose not work although when I load the qpcr product on gel, it appears positive, there is clear band on gel electrophoresis.
13 June 2016 8,793 7 View
can anybody suggest the best way of increasing the specificity of Ryan 2013 nasted primers for 18S rRNA of Cryptosporidium spp detection? in other word, that primer cross reacting with other...
01 June 2016 4,451 1 View
Is there anybody tried preparing intestinal tissue from rodent for identification of Cryptosporidium? please advise.
03 March 2016 6,503 3 View
has any body tried using Qaigen stool mini kit for extracting Cryptosporidium oocysts directly from feacal. IIf so, what is the concentration you usually getting since I have tried using it and it...
12 January 2016 7,757 4 View
Does anyone know why after extracting Cryptosporidium DNA from feacal samples, the first PCR works well but when I need to run another PCR from the same extracted DNA, it does not work on gel...
28 November 2015 3,981 4 View
DNA extraction from FTA descs
26 October 2015 6,067 2 View
Have anyone tried using FTA cards for Cryptosporidium DNA extraction? in other words, is it possible subjecting fecal samples to FTA cards? i hope to find answer to this question since my research...
22 April 2015 7,836 3 View
