Elise Bonnet

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Questions related from Elise Bonnet

What RPA amplicon to buffer ratio is suitable to detect RPA products using Abingdon Health PCRD Nucleic acid detector sticks?

I am currently developing RPAs for the detection of flaviviruses, however I am struggling a bit with determining the minimum detection limits of my assays. I performed ten-fold dilutions on...

10 October 2018 2,494 1 View

I am designing RPA primers, however I am struggling with multiple wobbles bases (I am using the most conserved gene). Please help?

I am using the most conserved gene (NS5) of flaviviruses. There are multiple wobble bases in potential primer sequences I am looking at, app. 10 at the most. Are there any rules concerning wobble...

01 January 1970 7,125 0 View