Elisabetta Ciani

3 Questions 2 Answers 0 Followers

Questions related from Elisabetta Ciani

Why might 293 HEK cells not be incorporating BrdU anymore?

BrdU immunocytochemistry is not working in the 293 HEK cells that we have in the lab. The method seems to be correct since in parallel in other cell lines, such as SH SY5Y cells BrdU staining is...

30 May 2014 4,836 3 View

How to store a purified protein before a kinase assay?

I need to store a protein, purified at different times, for a kinase assay. The protein purified from 293T cells is very dilute. How do you recommend storing? At -80 ° C, with or without glycerol,...

24 September 2013 8,812 1 View

Is it possible to position the HA taq not at the extreme end of a protein and visualize the taq-protein by Immunocytochemistry?

I’m trying to purify a fusion protein by two step purification STREP-6HIS. In my construct I would also like to introduce a HA taq because there are not good antibodies in my protein. I wonder if...

07 June 2013 6,032 9 View