Elisabetta Ciani

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Questions related from Elisabetta Ciani

How to store a purified protein before a kinase assay?

I need to store a protein, purified at different times, for a kinase assay. The protein purified from 293T cells is very dilute. How do you recommend storing? At -80 ° C, with or without glycerol,...

24 September 2013 8,733 1 View

Is it possible to position the HA taq not at the extreme end of a protein and visualize the taq-protein by Immunocytochemistry?

I’m trying to purify a fusion protein by two step purification STREP-6HIS. In my construct I would also like to introduce a HA taq because there are not good antibodies in my protein. I wonder if...

07 June 2013 5,981 9 View