Elisabetta Ciani

2 Questions 1 Answers 0 Followers

Questions related from Elisabetta Ciani

How to store a purified protein before a kinase assay?

I need to store a protein, purified at different times, for a kinase assay. The protein purified from 293T cells is very dilute. How do you recommend storing? At -80 ° C, with or without glycerol,...

24 September 2013 8,750 1 View

Is it possible to position the HA taq not at the extreme end of a protein and visualize the taq-protein by Immunocytochemistry?

I’m trying to purify a fusion protein by two step purification STREP-6HIS. In my construct I would also like to introduce a HA taq because there are not good antibodies in my protein. I wonder if...

07 June 2013 5,993 9 View