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Questions related from Elisabetta Ciani
BrdU immunocytochemistry is not working in the 293 HEK cells that we have in the lab. The method seems to be correct since in parallel in other cell lines, such as SH SY5Y cells BrdU staining is...
30 May 2014 4,836 3 View
I need to store a protein, purified at different times, for a kinase assay. The protein purified from 293T cells is very dilute. How do you recommend storing? At -80 ° C, with or without glycerol,...
24 September 2013 8,812 1 View
I’m trying to purify a fusion protein by two step purification STREP-6HIS. In my construct I would also like to introduce a HA taq because there are not good antibodies in my protein. I wonder if...
07 June 2013 6,032 9 View