Elisa Romeo

3 Questions 5 Answers 0 Followers

Questions related from Elisa Romeo

How to quantify fluorescent-labelled peptide?

Hi, I have two sets of peptides (4 to 6 amino acids), which are labelled with FITC and Cy5, respectively. I am trying to find a robust method to quantify their concentration. Currently, I am using...

01 July 2025 7,194 3 View

Why Lemo21 produce high amount of recombinant protein without induction?

Hi, I'm using Lemo 21 to express a protein in the periplasm. I'm trying three different constructs where my protein starts with three signal peptides. The sequences are cloned in pET28. I have...

05 March 2025 4,827 6 View

Tetrazine or methyl tetrazyne, which is best?

Hi all, I will start a project dealing with photo activity labeling using a compound functionalized with both diazirine (for photo-crosslinking) and cyclopropene (for inverse Diels–Alder tetrazine...

17 October 2018 1,892 2 View