Elif Bayraktar

5 Questions 3 Answers 0 Followers

Questions related from Elif Bayraktar

Is it possible to detect heterozygous deletions in IGV?

Hello, I am working on a case which patient is diagnosed with PD. In their WES results, a total homozygous deletion of 3rd and 4th exomes of the PARK2 gene is present. There is consanguinity in...

17 August 2018 4,425 4 View

What happened to the plasmid DNA?

Hello, I am currently working on plasmid cloning and today I got so confused about one of my colonies. After isolating their plasmids, NanoDrop results said that the concentration of DNA and...

05 June 2018 8,920 4 View

What might be the the reason for the outcome for transformation?

Hello everyone, I am currently working on a clonning experiment where I inserted my gene into a special vector and transformed it into DH5alpha competent cells. The problem is after translation...

03 May 2018 5,167 4 View

What happens when a signal peptide is translocated?

Well, let's think that I have a protein that will be transfered into ER and its signal peptide is on N terminus of the protein. I want to cleave the sequence and add it on the C-terminus. Where...

23 October 2017 6,572 2 View

Is it possible to add a treshold in a signaling pathway?

What I am trying to say is, instead of immediately giving a response when the material exists in the environment, independed of the concentration, can we make the cell concentration sensitive? For...

27 September 2017 7,064 1 View