Elham Lak Mazaheri

14 Questions 24 Answers 0 Followers

Questions related from Elham Lak Mazaheri

I have some questions about the interpretation of MLPA data?

Dear Respected Scientists, Can we trust the results if MLPA data from amniotic samples for SMA indicates a ligation failure warning but shows normal peak heights in GeneMarker software? We also...

27 November 2024 8,626 0 View

How can we distinguish maternal contamination from triploidy in QF.PCR analysis?

Hello dear scientists How can we distinguish maternal contamination from triploidy in QF-PCR analysis?

10 December 2023 9,552 2 View

Can the specificity of primers change over time or depending on the sample?

Hello dear scientists. Can the specificity of primers change over time or depending on the sample? It looks like the primers I used in my recent PCR to target exon13 of CFTR gene on chromosome 7,...

23 August 2023 9,919 5 View

Where can I find the Liz 1200size standard for Gene marker software?

Hello dear scientists Where can I find the Liz 1200 size standard for Gene marker software ?

19 July 2023 1,439 1 View

Where can I find a list of common cystic fibrosis mutations and their exon locations?

Hello dear scientists Ideas for good articles or databases on cystic fibrosis and common or potentially pathogenic variants are most welcome. And what strategies would you suggest for carrier...

23 June 2023 2,343 4 View

Where can I get medical genetic lab standard protocols for genetic disorder testing??

Hello, dear researchers I want to set up a test to diagnose Huntington's disease. I would appreciate any guidance on the database where I can find new standards and approved protocols for genetic...

01 April 2023 864 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

22 February 2021 9,446 3 View

Is it possible that formazan Crystal formation took more than four hours ?

Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...

21 October 2020 1,326 3 View

How should a stock solution and work solution with herbal water extraction be prepared?

How should a stock solution and work solution with tree pollens be prepared? Dear honored scientists, For an herbal extraction that contains multiple compositions (like different kinds of...

13 August 2020 8,835 7 View

What is sham in mtt assay?

Dear scientists, When should we have sham in mtt assay? What is the sham dose? How should we use its data for mtt assay analysis? I heard that if my drug solvent is PBS should take a dose of it...

13 August 2020 2,177 3 View

Some questions about suspension cells MTT assay protocol?

Hello, dear scientists Can I seed cells with treating doses of the drug, and after that, add mtt reagent without removing media? If I don't remove drug solution before adding mtt reagent, is it...

21 February 2020 7,598 7 View

How do we choose a proper concentration for the stock solution?

Hello, dear scientist, I want to use mtt assay to find ic50 of my substance. The substance hadn't tested on the cell line that I choose (PC12). I don't know how to prepare the stock solution.

24 January 2020 6,400 6 View

How many doses should we choose for finding the IC50 of a substance?

how many doses should we choose for finding the ic50 of a substance?

24 January 2020 9,166 2 View

Can I extract collagen type iv from eggshell and use it for attaching pc12 cells ?

Hello dear scientists I want to extract collagen type 4 and then attach pc12 cells to 96 well plates. could you please suggest me some good protocols.

03 December 2019 761 5 View