Elena Gorokhova

9 Questions 65 Answers 0 Followers

Questions related from Elena Gorokhova

Software for estimating uncertainty of population growth rate?

I am looking for a software (preferably freeware) that can be used for estimating uncertainty of population growth rate calculated from life table data for small invertebrates. Has to be something...

03 March 2018 7,647 5 View

DNA clean-up after Chelex extraction - any suggestions?

I wonder if anyone has a positive experience for improving DNA quality following extraction with Chelex? This method is great for improving the yield, particularly for samples low in DNA or...

09 September 2017 4,243 9 View

Why some samples generate virtually no clones whereas others make plenty?

This is likely to be a very basic question - I am sorry for asking it but will do that anyway:) We are trying to clone and sequence bacterial communities coming from different samples by using the...

11 November 2015 1,212 8 View

Can anyone help with the statistics for nested experimental design with few observations?

What would be an appropriate statistical analysis (if any) for the following experimental design (nested treatment, duplicate observations per treatment): Response variable (continous) was...

04 April 2014 7,696 7 View

Running ANOVA in Generalized Linear Models (GLM) module

Many stat. packages offer to use GLM (or GLZ in Statistica) for ANOVA analysis. My question is - when does it makes sense to use Generalized Linear Model to test for an effect of a single...

01 January 2014 2,725 16 View

Any advice for partial linear square regression in STATISTICA?

When running PLS with NIPALS in STATISTICA, I ran into a weird problem. In the module “Variable Importance”, the VIP values are never larger than 0.75 for any variables. To be acceptable as an...

12 December 2013 8,855 14 View

How to optimize mixed model for GLM with incomplete dataset for "before and after" analysis?

We have a dataset that is difficult to squeeze in the mixed model (glm). The dataset consists of 3 field stations where 4 species of benthic animals were sampled before and after an event over 2...

11 November 2013 5,497 13 View

SOD assay with cytochome c - uninhibited treatment is consistently lower than anything else - can anyone help?

When running a classical SOD (superoxide dismutase) assay, samples with xanthine (X) and xanthine oxidase (XOD) produce consistently low absorbance compared to the standards (X+XOD+SOD at...

10 October 2013 7,202 9 View

How to pellet an algal suspension?

What may sound like a very simple question is driving crazy 2 experienced lab technicians. We run growth inhibition tests with green algae using 5 ml incubation volume and low algal density. At...

09 September 2013 5,213 33 View