Elena Zaaba

7 Questions 10 Answers 0 Followers

Questions related from Elena Zaaba

No staining observed when slide is co-incubated with two different antibodies?

Hi, I am trying to incubate a rat pancreatic section with two different antibodies (co-incubation): 1. Anti pancreatic polypeptide (goat polyclonal) - did a solo staining and works on rat's...

19 October 2016 8,222 5 View

Is it necessary to keep gelatin coated slides (for IHC) in the fridge?

I have been given some blocks to be sectioned and some gelatin coated slides too. The predecessor of this project had kept both unused gelatin coated slides and sections at 4C. Is it necessary to...

17 February 2016 9,323 3 View

Low molecular weight proteins are not getting transferred! Help!!

Hi, I ran two gels couple of days ago and did not get good bands. I initially thought that it was my secondary that's not working well. But when I checked the gels I realized that there were thick...

10 February 2016 5,762 6 View

Can I reprobe a blot without stripping?

Hi, is it possible to reprobe a blot after visualizing it (by adding another primary antibody, secondary, ECL plus) without stripping it first? I was told by a colleague that I can can skip the...

27 January 2016 9,107 7 View

Can a blot be kept for a few days after secondary incubation?

Hi, I was wondering if a blot (western blot) can be kept in a fridge at 4C for a few days after secondary incubation? If so then should it be kept in TBST? I heard that over exposure with TBST...

25 October 2015 7,871 2 View

Can anyone help with the problem dissolving protein pellet in 1% SDS + 8M Urea?

Hi, I have been trying to dissolve retina and lens' protein pellets unsuccessfully for the past couple of weeks. Protein was extracted using trisol and everything went well until the dissolving...

11 October 2015 7,741 12 View

Problem dissolving 1g DAB powder in 40ml ddH2O?

Hi, I am trying to prepare DAB for IHC. I have recently purchased 5g of DAB http://www.sigmaaldrich.com/catalog/product/sigma/d5637?lang=en®ion=AE and according to our protocol, I need to...

01 January 1970 5,061 10 View