Elena S. Martynova

4 Questions 8 Answers 0 Followers

Questions related from Elena S. Martynova

How to improve sharpness of the bands in radioactive EMSA (gel shift)?

Hi everyone, Yes, I know, almost anyone nowadays is using radioactive labeled probes for EMSA, but since I was not lucky with fluorescent labeled probes I tried to do it old way. Can anyone please...

03 June 2016 562 3 View

Can you advice any commercially available kits for ChIP?

Hi guys,I always used to perform ChIP experiments using old fashioned protocol that takes as some of you might know up to 5 days till you get the results. I have seen there are plenty of...

16 January 2016 9,553 8 View

How can I fix low primer efficiency with SYBR green qPCR?

Hi everyone,Can anyone give me advice about how to improve my qPCR primer efficiency: I design primers using primer3 trying to get GC content around 55%. When I perform primer test using standard...

06 August 2015 5,526 8 View

Any advice on mouse embryonic stem cells establishment?

Hi everyone,I am new to mES field and my task is to establish mES cells from transgenic mice. I have already done it for C57Bl/6 mice but when I switch to C57Bl/6 transgenic mice I get some...

05 August 2015 915 5 View