Elena S. Martynova

3 Questions 7 Answers 0 Followers

Questions related from Elena S. Martynova

How to improve sharpness of the bands in radioactive EMSA (gel shift)?

Hi everyone, Yes, I know, almost anyone nowadays is using radioactive labeled probes for EMSA, but since I was not lucky with fluorescent labeled probes I tried to do it old way. Can anyone please...

03 June 2016 524 3 View

How can I fix low primer efficiency with SYBR green qPCR?

Hi everyone,Can anyone give me advice about how to improve my qPCR primer efficiency: I design primers using primer3 trying to get GC content around 55%. When I perform primer test using standard...

06 August 2015 5,480 8 View

Any advice on mouse embryonic stem cells establishment?

Hi everyone,I am new to mES field and my task is to establish mES cells from transgenic mice. I have already done it for C57Bl/6 mice but when I switch to C57Bl/6 transgenic mice I get some...

05 August 2015 862 5 View