Eleftheria Anastasopoulou

2 Questions 2 Answers 0 Followers

Questions related from Eleftheria Anastasopoulou

Any suggestions on stimulation of sorted memory T cells with peptide-pulsed matute DCs?

I am planning to investigate tumor-specific CD4 T cells upon in vitro expansion(10 days) with the cognate peptide. Is the ratio 1:5 (DC:T cells) sufficient? Apart from IL7 and IL2, should I also...

11 January 2016 9,459 1 View

Does anyone have experience with Antigen specific CD8 T cell detection using tetrameters?

I want to investigate antigen specific CD8 T cells using tetramers. Since these specific cells are rare in PBMCs, I am using in vitro stimulation. I want to ask if it is possible to isolate CD8+ T...

29 August 2014 8,729 6 View