Ekkaphot Khongkla

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Questions related from Ekkaphot Khongkla

Can we used serum-free medium instead of opti-MEM for lipofectamine transfection ?

I am afraid that some components loaded in Opti-MEM might affect the change in morphology of cells since I won't remove transfecting media out after transfection. I am thereby considering to...

21 October 2020 8,939 4 View

How much of GFP plasmid used for transfection?

I did lipofectamine 3000 tranfection in neuronal cell line. I used 2 ug of pmaxGFP. After the DNA-lipid complex was formed, it was added into cell for 3 h. Then transfected media was removed and...

01 January 1970 1,430 5 View