Ekaterina Kuznetsova

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Questions related from Ekaterina Kuznetsova

Any advice on ITC with low affinity compounds (low c-value titrations)?

I am working with several compounds that are expected to have a Kd of ~ 20 uM. They are also poorly soluble in aqueous solutions so it is not possible to titrate high concentration of compound...

31 January 2020 6,242 2 View

Hello all, I was wondering if anyone was able to reproduce inhibition of IDH2 R140Q by AGI-6780 as outlined in PMID:23558173?

We are following conditions outlined in the paper, long pre-incubation time and still there is barely any effect on enzyme activity. Publication claims nanomolar IC50, although I cannot find any...

05 August 2015 4,916 0 View