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We studied somatic mosaicism in neurons. We designed specific primers for a neuronal gene that should produce a long product (1700 bp) and performed a PCR with genomic DNA and LongAmp polymerase....
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Hello colleagues, I recently had much unspecific staining in my immunofluorescence assay. The blocking solution we use is not sterile and contains three different sources of protein in high...
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Hello colleagues, I have one dubious pair of primers for a snail gene which efficiency is very unstable and can oscillate from 80 to 110% in different reactions (standards are not to blame,...
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Hello everybody,I've had some problems with PCR efficiency a while ago (you can read the details here:...
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