Ekaterina Chesnokova

6 Questions 29 Answers 0 Followers

Questions related from Ekaterina Chesnokova

How it's possible that a PCR product is longer than the matrix?

We studied somatic mosaicism in neurons. We designed specific primers for a neuronal gene that should produce a long product (1700 bp) and performed a PCR with genomic DNA and LongAmp polymerase....

05 April 2021 7,158 1 View

Can bacterial growth in blocking solution cause unspecific binding of antibodies?

Hello colleagues, I recently had much unspecific staining in my immunofluorescence assay. The blocking solution we use is not sterile and contains three different sources of protein in high...

27 September 2018 1,555 6 View

Why do I see melt curve peak shifted to the left in more diluted samples (this is not a dimer peak)?

Hello colleagues, I have one dubious pair of primers for a snail gene which efficiency is very unstable and can oscillate from 80 to 110% in different reactions (standards are not to blame,...

06 August 2018 690 4 View

I compare two dilutions of the same sample with ddPCR and the difference is too big - how can it be?

Hello, I have a problem with ddPCR. Usually I dilute cDNA for it, to get rid of PCR inhibitors from revertase mix. But I was taught that inhibitors are less crucial for ddPCR than for RT-PCR...

06 February 2018 6,907 3 View

Is it crucial for TaqMan RT-PCR assay to specify the type of quencher in reaction setup?

Hello colleagues, I have designed some TaqMan probes for using in ddPCR, and to save expensive ddPCR reagents I decided to test the probes in conventional RT-PCR machine first. The probes are...

11 October 2017 8,550 5 View

How it's possible that overdiluted random primers used in RT can decrease PCR efficiency?

Hello everybody,I've had some problems with PCR efficiency a while ago (you can read the details here:...

03 February 2017 4,157 9 View