Ehsan Razmara

3 Questions 20 Answers 0 Followers

Questions related from Ehsan Razmara

Problem with huge mutation rate in Cloning ?

We have amplified a slightly large segment of K562 cDNA -2271bp- in E.Coli TOP10. At first, we’ve used of MaxTaq Enzyme (Vivantis Com.), but the mutation rate was so great and we found 5...

09 September 2017 6,720 6 View

What's the best source to check the gene expression in Ankylosing spondylitis ,PBMC , macrophage or monocyte ?

I would like to check the gene expression in the AS patients, but I don't know, I must measuring these genes in PBMC, macrophage or monocytes? Meanwhile, my mutant gene doesn't have any expression...

01 January 2017 3,035 2 View

How can I find STR sequences in database ? Is there any database to design a STRs primers for PND ?

I often use UCSC database but when i do it in PND i found it isn't correct and then I can't trust this database .Is it another Database to find a STRs primers ?

11 November 2016 4,737 2 View