Edward A Berry

6 Questions 14 Answers 0 Followers

Questions related from Edward A Berry

Predict solubility of organic compounds?

Is there a formula, or a program or web server, that will predict the solubility of an arbitrary organic compound in various solvents?

30 August 2018 6,395 3 View

Which isomer of tetramethylbenzidine (TMBZ) works best as a peroxidase substrate for detecting covalent heme in SDS gels?

Thomas et al. (Anal Biochem 75, 177) recommended using the 3,3',5,5'-tetramethyl isomer, but googling for online recipes shows a number of labs are using N,N,N',N'-tetramethyl. Do they both...

08 February 2017 6,830 2 View

Can His-tagged proteins be eluted from Ni-NTA by low pH instead of imidazole?

And if so, how low does the pH need to be to elute cleanly in a small volume?

02 September 2015 8,263 3 View

What is the source of varying levels of 2,3-BPG that regulate O2 affinity of HGb?

The affinity of hemoglobin for oxygen is regulated by varying concentration of BPG (2,3-bisphosphoglycerate), which binds to and stabilizes the deoxy form. BPG is a side product of glycoloysis....

24 August 2015 4,651 7 View

Are there bifurcated H-bonds in the transition of a protein helix from alpha to 3-10?

Sometimes alpha helices have segments of 3/10 helix at the end. In the transition area, there are residues for which the amide N is equally close to the carbonyl oxygens of the residues 3 and 4...

08 April 2015 6,285 2 View

Should BHT be added to ether containing high level of peroxide?

Hypothetical question here- If a bottle of diethyl ether or dioxane is found to have a dangerous level of peroxide, should a radical scavenger/antioxidant like BHT be added to reduce the risk of...

08 April 2015 3,882 3 View