Eduardo Reyes-Serratos

2 Questions 2 Answers 0 Followers

Questions related from Eduardo Reyes-Serratos

Which is the best protein assay to determine the concentration in saliva supernatant?

I have been using a Pierce BCA Protein Assay kit (Thermo Scientific) and diluting my samples (saliva:diluent) 1:10, 1:20, 1:40. I do a triplicate of each dilution. The variation in between...

20 March 2018 5,125 2 View

After thawing an aliquot of the antibody I am using (aliquots are stored at -80°C), I place the remaining volume at 4°C for later reuse. Is this good?

I have a limited volume of a polyclonal antibody and I look to use it as efficiently as possible. I have aliquoted the antibody and stored the aliquots at -80°C. Whenever I need to perform an...

29 October 2017 9,735 7 View