Ebru Aras

3 Questions 1 Answers 0 Followers

Questions related from Ebru Aras

My housekeeping gene has higher CT values than the interested samples. How can I overcome this issue?

I have been doing some qPCRs on bacterial genomic DNA. I am using 16S as my "housekeeping gene". My sample of interest, however, yield a lower CT value. I am trying to figure out how to overcome...

21 April 2023 2,096 3 View

How can I isolate RNA and synthesis cDNA from mouse serum samples?

I obtained mouse serum samples. My initial plan is to check some proteins using ELISA. However, plans have changed and now I need to check some gene expressions. My questions are: 1) Can I...

09 June 2021 791 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

19 February 2021 7,119 3 View