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Questions related from Dushyant Kumar Garg
In the last decade, after realization that even short amino acid stretches can undergo amyloidogenesis, numerous short peptides of varying length were studied for their amyloid forming propensity....
16 January 2017 6,241 3 View
We studied guanidinium hydrochloride (GdnCl) mediated equilibrium unfolding of a multidomain dimeric hyperthermophilic protein (each subunit consists of 2 domains). Upto 1M GdnCl, protein exhibits...
26 March 2016 4,354 7 View
My experiment entails studying biotin-streptavidin interaction through SPR. I am intending to immobilize streptavidin over a 50 nm thick gold layer. The gold layer is pre-formed on a bare core of...
28 January 2016 4,341 6 View
I want to study the stability of one dimeric hyperthermophillic protein at different pH. One way of doing so is to subject pH incubated protein sample to thermal scanning and determine Tm. The...
25 December 2014 1,913 3 View
I am working on a hyperthermophilic protein which is dimeric in its native state. I am checking at what guanidine concentration the dimer dissociates into a monomer. One way to do so is to employ...
18 June 2014 5,399 6 View