Dushyant Kumar Garg

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Questions related from Dushyant Kumar Garg

Why short peptides of six amino acid length (hexamer) are preferred choice to study amyloid formation?

In the last decade, after realization that even short amino acid stretches can undergo amyloidogenesis, numerous short peptides of varying length were studied for their amyloid forming propensity....

16 January 2017 6,270 3 View

How to compare the protease sensitivity of a protein through limited proteolysis that is carried out at different pH?

We are working on a protein of hyperthermophilic origin that remains in dimeric state at native pH ( pH 8.0). It dissociates into folded monomers at pH 2.0. The protein is kinetically stable in...

06 December 2016 7,163 3 View

Protein undergoes initial compaction and structural gain at low guanidine HCl concentration before unfolding at higher GdnCl concentration

We studied guanidinium hydrochloride (GdnCl) mediated equilibrium unfolding of a multidomain dimeric hyperthermophilic protein (each subunit consists of 2 domains). Upto 1M GdnCl, protein exhibits...

26 March 2016 4,382 7 View

How to conjugate streptavidin over a gold layer so as to obtain self assembled layer (SAM) of streptavidin?

My experiment entails studying biotin-streptavidin interaction through SPR. I am intending to immobilize streptavidin over a 50 nm thick gold layer. The gold layer is pre-formed on a bare core of...

28 January 2016 4,365 6 View

How do I make sure that the pH of my protein sample is not altered at different guanidine concentration?

I want to study the stability of one dimeric hyperthermophillic protein at different pH. One way of doing so is to subject pH incubated protein sample to thermal scanning and determine Tm. The...

25 December 2014 1,939 3 View

What is the correct way of calculating percentage protein unfolding by intrinsic trp fluorescence?

While calculating the percentage protein unfolding through equilibrium unfolding by intrinsic tryptophan fluorescence method, people generally plot intensity at single wavelength (e. g. 340nm)...

31 August 2014 5,420 6 View

How can I calculate the dn/dc value for a protein in buffers containing high concentrations of urea/guanidine hydrochloride?

I am working on a hyperthermophilic protein which is dimeric in its native state. I am checking at what guanidine concentration the dimer dissociates into a monomer. One way to do so is to employ...

18 June 2014 5,427 6 View