Dulshara Sachini Amarasekara

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Questions related from Dulshara Sachini Amarasekara

Any one experienced in using pSico vector based Lentiviral knockdown system?

I am using the pSico based shRNA knockdown system to knock down my protein of interest in HT-29 cells. But the western results show lower or no expression of the protein for the vector only(pSico)...

07 July 2017 3,944 2 View

Has anyone use gp2-293 based retro virus to infect caco-2 or HT-29 cells? Any suggessions for better results?

I want ro try retro viral gene transfer first and then move to lenti viral system if it doesnt work well. Since gp2-293is known to produce amphotropic viruses which can infect human cells as well,...

02 February 2017 3,420 3 View