Dr. Ruby Bansal

3 Questions 11 Answers 0 Followers

Questions related from Dr. Ruby Bansal

Why ITC thermograph shows dip?

I am studying a Protein interaction with metal ion and during titration I got the graph attached below. The concentration of protein used was 0.09mM and metal ions concentration was 1 mM. Could...

07 July 2017 5,205 19 View

What points I should consider or take care of in order to get reproducible results in ITC experiments of protein study

I did ITC experiments with BSA and Its difficult to get same results with same concentration of protein as well as binding ligand.

03 March 2017 6,376 8 View

Could anyone help me in studying aggregation of protein using ITC?

We have been using Microcal ITC for studying protein aggregation but could not able to get any trend in results and interpreting graphs as well. Does someone has any idea how aggregation of...

01 January 2017 7,513 0 View