Dr. Narendra Kumar Sharma

7 Questions 17 Answers 0 Followers

Questions related from Dr. Narendra Kumar Sharma

Methods for Biochemical analysis (GSH, GSSG, Cat, SOD, ROS, MDA and antioxidant assays) from animal tissue samples?

Hi, we are working on antioxidants and routinely use these parameters with old referenced articles. We have observed the several limitations of these conventional methods and still want to use...

09 August 2024 8,066 3 View

How can I control FDR in PLGS, in DDA mode?

Hi, I am trying to analyse proteomic data in PLGS from waters. I have quantitative data, so need to process in DDA mode but in workflow parameters, there was no option for FDR. Can any one suggest...

19 March 2015 2,752 3 View

How can we optimize conditions for iTRAQ 8 plex for depleted plasma by Waters SYNAPTH G2 HDMS?

If some one can share their experience using the same instrument for 8 plex iTRAQ experiments like energy parameters, scan timing etc

01 December 2014 690 1 View

Anyone have idea how to find a miRNA target site?

I wants to find miRNA target site by manual or tool available foe access

12 September 2014 5,900 14 View

Is a cut-off filter suitable to exchange buffer from protein samples?

I experienced, that when I used 3 or 5 kd cut-off filter tubes to exchange buffer for proteomics work, the flow through have some protein content. Theoretically it should not have happened. In...

30 January 2014 2,198 2 View

Is it necessary to acetone precipitate plasma proteins before iTRAQ labelling for mass spec?

As Plasma has high amount of salt and lipids, what is the best methodology for sample preparations before labelling?

28 January 2014 6,697 3 View

How can we inhibit a particular signaling pathway?

To silence genes of a particular pathway to study the significance of said pathway.

20 January 2014 5,058 9 View