Donald T McKnight

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Questions related from Donald T McKnight

Is Greengenes or SILVA better for bacterial microbiome studies?

Greetings fellow microbial researchers, do you prefer to use Greenegenes or SILVA as a reference database for bacterial (16S) microbiome studies? Why? One obvious advantage of SILVA is that it has...

24 April 2017 8,968 7 View

Best tissue type for DNA extraction from formalin fixed samples

I am about to attempt to get genomic DNA (for genotyping by sequencing) from frogs that were fixed in formalin and subsequently stored in EtOH, and I was wondering if anyone had recommendations on...

06 March 2017 9,658 1 View

Cause of double bands in fungal microbiome extraction/PCR?

I’ve been trying to simultaneously extract bacterial DNA and fungal DNA from swabs of skin samples, but I seem to be having some form of contamination. This may take a minute to explain, but let...

14 February 2017 3,884 4 View