Dominique Massau

4 Questions 2 Answers 0 Followers

Questions related from Dominique Massau

Which factors have a major influence on events leading towards FPLC?

Hello, I'm currently trying to purify a his-tagged protein with AC. However, when running the HPLC I have a very dissapointing protein yield (both total protein and the protein of...

12 December 2016 7,403 7 View

How can I prepare a 1M potassium phosphate buffer with a pH of 7,8?

I have found an online protocol on how to make the buffer, but I am not sure about the calculations. Could anyone give me a procedure?

11 November 2016 1,319 5 View

Is it possible to use a cadmium column to purify a protein?

I'm doing a project on optimalization of protein isolation and purification. I have heard from a colleague that the yield of the protein is greater when using a cadmium column compared to a nickel...

11 November 2016 4,589 5 View

How can I prepare a 1M potassium phosphate buffer with a pH of 7,8?

I have found an online protocol on how to make the buffer, but I am not sure about the calculations. Could anyone give me a procedure?

06 November 2016 3,397 4 View