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These are normal human fibroblasts derived from foreskin. We ordered them from ATCC. I tried transfecting them with a generic GFP expressing vector using both Turbofect and Transit X2 and got...
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We want to stain the nucleus in live cells. I know that DRAQ5 can label DNA in live cells but I've heard that it can be toxic. I'd appreciate any experiences others might have had using this stain...
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I've made a targeted gene insertion into the genome of mammalian cells using ZFN technology. The insertion is a fluorescent protein at the C-term of my GOI. I have validated the genomic dna by...
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The pTandem1 vector from Novagen has a CMV promoter, exon1, exon2 (consisting of a bunch of tags followed by and IRES sequence and sprinkled with restriction sites for subcloning GOI). Exon1 and...
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