Dmitri Samovski

4 Questions 5 Answers 0 Followers

Questions related from Dmitri Samovski

Crosslinking of proteins in tissue lysate for co-Immunopercipitation?

Greetings, I am trying to detect transient protein-protein interactions in tissue lysates from mouse skeletal muscle. I have tried co-IP, and even though I am able to get a good IP of my...

11 November 2017 7,251 0 View

Does anyone know a good mice model for diet-induced NASH?

I am looking for an efficient diet to induce liver steatosis in mice, preferably without the use of a liver toxin. I know that it is hard to induce liver disease in mice and was looking to see if...

02 February 2016 1,263 2 View

PGC1a phosphate antibodies?

Does any know of a commercially available antibody for PGC1a AMPK-phosphorylated sites, as described in http://www.ncbi.nlm.nih.gov/pubmed/17609368. I was looking for one online, but could not...

02 February 2014 5,715 0 View

Will sterilization of collagen coated cell-culture plates by UV light effect cell adherence to the plates?

I am culturing C2C12 myotubes for immunofluorescence on collagen-coated plates and wondered if I can sterilize the plates after the collagen is dry with UV light? Will it damage the collagen, so...

06 June 2013 10,137 9 View