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Questions related from Dmitri Samovski
Greetings, I am looking for your input on the following question: We are trying to design a study where we will inject sEVs (exosomes) isolated from human plasma and tissue biopsies to mice to...
09 February 2020 7,790 3 View
We are trying to reduce non-specific background for fluorescent (IR) WB. I know we used to wet the PVDF membranes before the transfer. There is no wetting step in the instructions for the...
27 December 2017 8,634 2 View
Greetings, I am trying to detect transient protein-protein interactions in tissue lysates from mouse skeletal muscle. I have tried co-IP, and even though I am able to get a good IP of my...
11 November 2017 5,798 2 View
Greetings, I am looking at phosphorylation of Akt (S473) in response to insulin. In addition to the increase in pAKT, the total Akt band also appears much bigger, even though the loading controls...
11 July 2016 7,070 8 View
I am looking for a way to detect Phosphatidylinositol (3,4,5)-trisphosphate levels in hearts of mice. All I can find are methods that employ cultured cells. Is there a reliable method to extract...
20 April 2014 6,963 6 View
Does any know of a commercially available antibody for PGC1a AMPK-phosphorylated sites, as described in http://www.ncbi.nlm.nih.gov/pubmed/17609368. I was looking for one online, but could not...
05 February 2014 8,145 0 View
I was wondering if anyone looked at AMPK activation (phosphorylation of T172) in-vivo in skeletal muscle or heart after overnight fasting. I would expect it would be activated, but I could not...
17 September 2013 7,977 3 View
I have recently started working with 3T3-L1 cells. When I start the differentiation of the cells using the standard protocol they look like they differentiate fine (they round up and accumulate...
21 August 2013 2,979 26 View
I am trying to measure fatty acid oxidation in cultured myotubes. I saw that in many studies people add carnitine to their buffer. I wonder why do they do that? Is there not enough carnitine in...
11 June 2013 9,226 6 View