Dmitri Samovski

9 Questions 11 Answers 0 Followers

Questions related from Dmitri Samovski

Can you inject human exosomes to mice?

Greetings, I am looking for your input on the following question: We are trying to design a study where we will inject sEVs (exosomes) isolated from human plasma and tissue biopsies to mice to...

09 February 2020 7,791 3 View

Does Immobilon-FL PVDF Membrane require wetting in methanol?

We are trying to reduce non-specific background for fluorescent (IR) WB. I know we used to wet the PVDF membranes before the transfer. There is no wetting step in the instructions for the...

27 December 2017 8,637 2 View

Crosslinking of proteins in tissue lysate for co-Immunopercipitation?

Greetings, I am trying to detect transient protein-protein interactions in tissue lysates from mouse skeletal muscle. I have tried co-IP, and even though I am able to get a good IP of my...

11 November 2017 5,801 2 View

Would phosphorylation of protein make the bands on WB gel look bigger when looking at the total levels of the same protein?

Greetings, I am looking at phosphorylation of Akt (S473) in response to insulin. In addition to the increase in pAKT, the total Akt band also appears much bigger, even though the loading controls...

11 July 2016 7,076 8 View

How to detect PIP3 levels in tissues ?

I am looking for a way to detect Phosphatidylinositol (3,4,5)-trisphosphate levels in hearts of mice. All I can find are methods that employ cultured cells. Is there a reliable method to extract...

20 April 2014 6,965 6 View

PGC1a phosphate antibodies?

Does any know of a commercially available antibody for PGC1a AMPK-phosphorylated sites, as described in http://www.ncbi.nlm.nih.gov/pubmed/17609368. I was looking for one online, but could not...

05 February 2014 8,148 0 View

Would you expect AMPK activation in skeletal muscle after overnight fasting in rodents or humans?

I was wondering if anyone looked at AMPK activation (phosphorylation of T172) in-vivo in skeletal muscle or heart after overnight fasting. I would expect it would be activated, but I could not...

17 September 2013 7,980 3 View

3T3-L1 adipocytes "peel off" around the edges of the plate?

I have recently started working with 3T3-L1 cells. When I start the differentiation of the cells using the standard protocol they look like they differentiate fine (they round up and accumulate...

21 August 2013 2,981 26 View

Why do you need to add carnitine when measuring fatty acid oxidation in cultured myotubes?

I am trying to measure fatty acid oxidation in cultured myotubes. I saw that in many studies people add carnitine to their buffer. I wonder why do they do that? Is there not enough carnitine in...

11 June 2013 9,230 6 View