Divya Nedungadi

4 Questions 4 Answers 0 Followers

Questions related from Divya Nedungadi

Are there any differences between the primers used for reverse trancriptase pcr and that used for real time q pcr?

Refering a paper we designed primers and did not notice that they used it for RT-PCR . While we tried RT-q PCR with it we did not get any proper amplification with the primers. This made us wonder...

10 October 2017 8,024 6 View

Cant we detect ubiquitinated proteins without their enrichment in the lysate?

ubiquitin antibody from cell signaling(3933), source rabbit,1:1000 dilution

10 October 2014 6,099 7 View

Do chemotherapeutic drugs not affect normal cells?

Why is it that when papers show the discovery of an anticancer agent, they show that it specifically affects only the cancer cells and not the normal cells? When we give a toxic substance like a...

05 May 2014 8,413 28 View

Why is my Qvd oph not working?

I tried qvd oph at a concentration of 20uM (1hr pre treatment) and then added an apoptosis inducing agent, but both my drug treated and qvd+ drug treated cells look the same. I am not able to...

05 May 2014 5,794 1 View