Divya Rana

7 Questions 19 Answers 0 Followers

Questions related from Divya Rana

Is Pluriselect a good company to buy kits for cell separation?

I am recently buying kits from Pluriselect to take out (deplete) NK cells and CD4 cells from blood. The main reason me using this is cheap and do not require accessory equipment. But in Pubmed...

03 May 2024 5,714 1 View

Sample size calculation for validating a new test against gold-standard?

Dear Friends! The gold-standard for identifying an allele for human leukocyte antigen is SBT or sequence-based typing. If I devise a new PCR test or LAMP test, how many known positive and negative...

17 February 2024 7,890 1 View

Are there commercial companies/services that carry immunology or molecular biology or animal experiment for you?

Sometimes it may be difficult or expensive to carry experiments by yourself. If commercial companies can do that at a satisfactory cost, our ideas can be translated into experimental results...

06 March 2023 5,141 4 View

Dear Researchers! I would be grateful if you please spend some time to help me understand, Can Sanger sequencing give data on biallelic human genes?

My specific question is, if (universal) primer is chosen and PCR amplification made for dizogitc organizims like human, both maternal and paternal chromosomal gene will be amplified. If both...

06 May 2022 942 7 View

Can an insignificant crude odds ratio in univariate analysis come significant in adjusted odds ratio during binomial logistic regression?

I found that a chi-square or odds ratio was not significant for a 2x2 table. Later when the independent variable was put together with other variables in binomial logistic regression in R, then...

24 February 2021 8,928 5 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

17 August 2020 8,535 2 View

Any advice on freezing the blood including plasma in the field?

We want to do whole blood assay in the field. Blood in cryovial will be stimulated with antigens. The other day, the tube with clot at below and the supernatant above is to be frozen UPRIGHT and...

28 July 2015 4,029 10 View