Divya Dinesh

5 Questions 7 Answers 0 Followers

Questions related from Divya Dinesh

Why my protein sample is not able to resolve well in SDS-PAGE gel?

When I run my protein lysate in 10% sds-page gel, while loading protein samples it coagulates and does not resolves properly in gel giving a smeared ponceau stain as shown in the photo attached....

14 May 2025 5,253 3 View

Why my protein of interest is not expressing in bacterial host cell?

I have cloned a gene pair in the pRSFDuet-1 vector. I have sequenced the clone. There is no issue with the gene sequence. The reading frame is also correct. I have tried to express the protein in...

08 April 2025 468 9 View

Why does my pdbsum generated file not showing interacting residues of two proteins?

I have uploaded docked complex of two proteins in pdbsum server. Both the proteins have different chain names in the pdb file. However, the pdbsum generated file is not showing protein-protein...

08 January 2025 2,225 0 View

Why PCR product not obtained at the desired size?

I have standardized the new primer set at specific conditions and obtained the PCR product at the desired size of ~360bp. However, when I repeated the PCR to that particular condition, I got a...

20 December 2024 325 7 View

Why vector backbone has a low intensity comparing to the insert after restriction digestion?

I have used a vector of 3.8kb and my insert is of size ~370bp. I have confirmed the vector size with single digestion before proceeding with the cloning process. I have isolated the plasmid from...

28 November 2024 7,376 2 View