Dina Nahhas

4 Questions 2 Answers 0 Followers

Questions related from Dina Nahhas

Why I cannot see clear proteins bands of Staphylococcus aureus with SDS page?

I am analysing the internal proteins of Staphylococcus aureus. I tried three different methods for extraction of the proteins prior loading on SDS page. I am growing the bacteria to log phase 0.4...

07 December 2021 2,035 5 View

Which medium is better to grow wild type Staphylococcus aureus; RPMI or TBS for skin infection proteomic analysis and why?

I am confused between both. TBS is widely used but RPMI depleted factors for growth is also considered in recent articles. Thanks for clarifications.

21 September 2021 6,044 0 View

What are the stress limiting conditions to grow clinical isolated S. aureus from skin infection in vitro to mimic its growth in vivo?

I want to cultivate S. aureus in TSB for proteome analysis and I want to consider any stressful factors during its growth on skin. I have not found any. Any suggestions that I can look up?

20 September 2021 5,287 0 View

RPM degree to grow S. aureus

I know that in most of the cases the RPM chosen to shake the bacteria while growing is overall 180. I grew S.aureus with 120 rpm and now I need to do replicates. I am hesitating with RPM. shall I...

01 January 1970 7,993 2 View