Dilan Dissanayake

10 Questions 14 Answers 0 Followers

Questions related from Dilan Dissanayake

What could be the reasons for discrepancies in stationary R-squared and MAPE?

Attached is a Table summarizing the MAPE and stationary R-squared values obtained for alternative exponential smoothing forecasting models for different variables. In general, the model with the...

09 May 2020 749 2 View

What is the criterion to determine the minimum number of lags required to make a meaningful forecasting using ACF?

I hope to forecast swine population using last 30 years of census data. The original time series has 81 time points (from 1937-2017) and I have already worked out the ACF correlogram (attached...

16 December 2019 3,740 3 View

What is the best model from the following exponential smoothing models?

I have modeled four non-seasonal exponential models using time series data on pork production over the period 1978-2012. data from 2013-2017 were hold-out for model validations. For simple...

01 June 2019 2,253 4 View

How to extract the plasma membrane fraction of cultured L6 muscle cells?

I want to separate the plasma membrane fraction from the cultured muscle cells upon sample treatment in order to analyze Glut4 translocation into the plasma membrane. I usually extract the cells...

15 June 2017 936 2 View

What is the best solvent to dissolve insulin powder from bovine pancreas when insulin-stimulated glucose uptake is assayed in cultured myotubes?

I am looking for the best solvent to dissolve insulin powder from bovine pancreas in order to incubate cultured myocytes when the glucose uptake is assayed. I usually dissolve insulin in 100% DMSO...

15 November 2016 8,137 2 View

Why Insulin Receptor Beta is used as the internal standard to determine GLUT4 level?

When investigating the underlying mechanisms of glucose uptake promoting effects, the GLUT4 levels of plasma membrane (PM) and cell lysate (CL) are determined in order to assess the GLUT4...

08 September 2016 5,228 6 View

What is the physiological charge of nicotinamide/niacinamide?

The pKa (strongest acidic) of nicotinamide/niacinamide has been given as 13.39 which should logically exist as a positively charged molecule at the physiological pH (7.4). However, the...

31 May 2016 7,126 1 View

Can anybody tell me how I can separate Gly-Tyr and Tyr-Gly with the same m/z using RP-HPLC?

I need to separate a mixture of dipeptides Gly-Tyr and Tyr-Gly using RP-HPLC.

05 January 2015 1,514 7 View

Dairy Microbiology - What should be the average value that I should use in cfu/mL?

I cultured Lactococcuc lactis subsp. lactis on M17 Media and obtained 6 x 106, 5 x 106 and 3 x 106 cfu/mL in three replicates. What should be the average value that I should use?

06 August 2014 3,451 7 View

What is the reason of observing a lag-phase in a pure Sacchoromyces cerevisiae culture cultured in milk?

Dairy Microbiology

25 July 2014 968 3 View