Dieter Kahl

3 Questions 4 Answers 0 Followers

Questions related from Dieter Kahl

Why did a RNase A treatment increase my Qubit measured sample RNA concentration?

I have diluted crude grapevine leaf total nucleic acid extracts. I quantified the RNA in five of these extracts using a Qubit RNA BR Assay Kit and found all of these diluted samples had RNA...

01 January 2020 2,709 5 View

Does cresol red interfere with Pfu polymerases?

I've used cresol red for years in routine Taq-based PCR's. I've never used it with high-fidelity systems such as Phusion. I can only find information online stating its compatibility with Taq...

05 May 2019 2,148 4 View

At what point does UNG/UDG become noticeably detrimental in one-step RT-PCR for the purpose of diagnostics?

I noticed we've been using a PCR system in our one-step RT-PCRs that incorporates dUTPs and uracil-DNA glycosylase (UDG). The UDG is not the cod-derived version. Our reverse transcription (RT'n)...

11 November 2018 8,979 0 View