Diane Squillace

2 Questions 1 Answers 0 Followers

Questions related from Diane Squillace

I would like to get feed back on the WES proteinsimple system and how it compares to older Western blotting technology?

We currently use NuPage mini gels (Bis-Tris or Try-Glycine). Our western blot apparatus is the old Bio-Rad mini-transblot cell. Our protocols vary from 1 hour tranblots to overnight. The problems...

18 July 2016 6,443 1 View

How can I extract tissue from frozen blocks (OCT compound) for protein analysis and ELISA?

I will be homogenizing the tissue in PBS with protease inhibitors and collecting supernatant for protein analysis and ELISA.

25 January 2016 9,705 3 View